TY  - JOUR
A1  - Francesconi, Caterina
A1  - Boštjančić, Ljudevit Luka
A1  - Rutz, Christelle
A1  - Hoffbeck, Lucien
A1  - Poidevin, Laetitia
A1  - Kress, Arnaud
A1  - Jussila, Japo
A1  - Makkonen, Jenny
A1  - Feldmeyer, Barbara
A1  - Bálint, Miklós
A1  - Schwenk, Klaus
A1  - Lecompte, Odile
A1  - Theissinger, Kathrin
T1  - Dataset of the de novo assembly and annotation of the marbled crayfish and the noble crayfish hepatopancreas transcriptomes
T2  - BMC Research Notes
N2  - Objectives: Crayfish plague disease, caused by the oomycete pathogen Aphanomyces astaci represents one of the greatest risks for the biodiversity of the freshwater crayfish. This data article covers the de novo transcriptome assembly and annotation data of the noble crayfish and the marbled crayfish challenged with Ap. astaci. Following the controlled infection experiment (Francesconi et al. in Front Ecol Evol, 2021, https://doi.org/10.3389/fevo.2021.647037), we conducted a differential gene expression analysis described in (Boštjančić et al. in BMC Genom, 2022, https://doi.org/10.1186/s12864-022-08571-z) Data description: In total, 25 noble crayfish and 30 marbled crayfish were selected. Hepatopancreas tissue was isolated, followed by RNA sequencing using the Illumina NovaSeq 6000 platform. Raw data was checked for quality with FastQC, adapter and quality trimming were conducted using Trimmomatic followed by de novo assembly with Trinity. Assembly quality was assessed with BUSCO, at 93.30% and 93.98% completeness for the noble crayfish and the marbled crayfish, respectively. Transcripts were annotated using the Dammit! pipeline and assigned to KEGG pathways. Respective transcriptome and raw datasets may be reused as the reference transcriptome assemblies for future expression studies.
KW  - Freshwater crayfish
KW  - Astacus astacus
KW  - Procambarus virginalis
KW  - Crayfish plague
KW  - RNA sequencing
Y1  - 2022
UR  - https://kola.opus.hbz-nrw.de/frontdoor/index/index/docId/2361
UR  - https://nbn-resolving.org/urn:nbn:de:kola-23611
ER  -