This study was conducted in Nyungwe National Park (NNP); a biodiversity hotspot Mountain rainforest of high conservation importance in Central Africa, but with little knowledge of its insect communities including butterflies, good indicators of climate change, and forest ecosystem health. The study aimed at availing baseline data on butterfly species diversity and distribution in NNP, for future use in monitoring climate change-driven shifts and the effects of forest fragmentation on the biodiversity of Nyungwe. Butterflies were collected seasonally using fruit-baited traps and hand nets along elevational transects spanning from 1700 m up to 2950 m of altitude. Two hundred forty-two species including 28 endemics to the Albertine Rift and 18 potential local climate change indicators were documented. Species richness and abundance declined with increasing elevation and higher seasonal occurrence was observed during the dry season. This was the first study on the spatial and temporal distribution of butterflies in NNP and further studies could be conducted to add more species and allow a depth understanding of the ecology of Nyungwe butterflies.