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Background: For over a century, scientists have studied host-pathogen interactions between the crayfish plague disease agent Aphanomyces astaci and freshwater crayfish. It has been hypothesised that North American crayfish hosts are disease-resistant due to the long-lasting coevolution with the pathogen. Similarly, the increasing number of latent infections reported in the historically sensitive European crayfish hosts seems to indicate that similar coevolutionary processes are occurring between European crayfish and A. astaci. Our current understanding of these host-pathogen interactions is largely focused on the innate immunity processes in the crayfish haemolymph and cuticle, but the molecular basis of the observed disease-resistance and susceptibility remain unclear. To understand how coevolution is shaping the host’s molecular response to the pathogen, susceptible native European noble crayfish and invasive disease-resistant marbled crayfish were challenged with two A. astaci strains of different origin: a haplogroup A strain (introduced to Europe at least 50 years ago, low virulence) and a haplogroup B strain (signal crayfish in lake Tahoe, USA, high virulence). Here, we compare the gene expression profiles of the hepatopancreas, an integrated organ of crayfish immunity and metabolism.
Results: We characterised several novel innate immune-related gene groups in both crayfish spe cies. Across allchallenge groups, we detected 412 differentially expressed genes (DEGs) in the noble crayfish, and 257 DEGs in the marbled crayfish. In the noble crayfish, a clear immune response was detected to the haplogroup B strain, but not to the haplogroup A strain. In contrast, in the marbled crayfish we detected an immune response to the haplogroup A strain, but not to the haplogroup B strain. Conclusions: We highlight the hepatopancreas as an important hub for the synthesis of immune molecules in the response to A. astaci. A clear distinction between the innate immune response in the marbled crayfish and the noble crayfish is the capability of the marbled crayfish to mobilise a higher variety of innate immune response effectors.
Objectives: Crayfish plague disease, caused by the oomycete pathogen Aphanomyces astaci represents one of the greatest risks for the biodiversity of the freshwater crayfish. This data article covers the de novo transcriptome assembly and annotation data of the noble crayfish and the marbled crayfish challenged with Ap. astaci. Following the controlled infection experiment (Francesconi et al. in Front Ecol Evol, 2021, https://doi.org/10.3389/fevo.2021.647037), we conducted a differential gene expression analysis described in (Boštjančić et al. in BMC Genom, 2022, https://doi.org/10.1186/s12864-022-08571-z) Data description: In total, 25 noble crayfish and 30 marbled crayfish were selected. Hepatopancreas tissue was isolated, followed by RNA sequencing using the Illumina NovaSeq 6000 platform. Raw data was checked for quality with FastQC, adapter and quality trimming were conducted using Trimmomatic followed by de novo assembly with Trinity. Assembly quality was assessed with BUSCO, at 93.30% and 93.98% completeness for the noble crayfish and the marbled crayfish, respectively. Transcripts were annotated using the Dammit! pipeline and assigned to KEGG pathways. Respective transcriptome and raw datasets may be reused as the reference transcriptome assemblies for future expression studies.
In Western personnel psychology, control beliefs are a valued predictor for work-related outcomes. Yet, little is known about the culture-specific functioning of control in East Africa. Kenya, as an Ubuntu culture, is examined regarding control beliefs and contrasted with a German sample considered to represent an individualistic or Western culture. Responses to N=143 quantitative personality tests were attended with qualitative interviews on control beliefs (self-concept of ability, internality, powerful others, and chance). Content validity and factor structure of control beliefs were analyzed, followed by a Procrustean target rotation. Linear regression analyses were conducted to assess the predictability of job performance, achievement motivation, and well-being. Item comprehension, as well as factor structure of the four control aspects, differ between the two samples. In particular, the ‘powerful others’ control aspect diverges the most between the cultures. Linear regression analyses showed comparable, but not fully congruent predictability. Results indicate that an uncritical transfer of the control beliefs measure from one culture to another is inappropriate. Results fit in the picture of African Ubuntu philosophy, emphasizing social-relational aspects shaping control beliefs. More emic-etic based research is demanded concerning intra- and intercultural variability of control beliefs to depict a transcultural applicable and invariant model.
Ponds in agricultural landscapes are often used by amphibians as breeding habitat. However, the characteristics of agricultural ponds and especially the surrounding area are usually said to be suboptimal for many amphibian species. Using suboptimal habitats might allow a species’ survival and reproduction, but can have negative consequences at the individual and population level. In the present study, we investigated Palmate Newt (Lissotriton helveticus) populations from an intensive wine-growing region in southern Germany and compared them with populations located in a nearby forested area in terms of biometric traits, age and genetic structure. By analyzing over 900 adult newts from 11 ponds, we could show that newts reproducing in forest ponds were larger than newts reproducing in agricultural ponds. We did not find differences in the newt age and growth rate between habitat types. Therefore, differences in the body size of newts might already existed in larvae and/or juveniles, what might be related to a lower habitat quality for larvae and/or juveniles in the agricultural landscape. Body mass, body condition and sexual dimorphic traits (length of the caudal filament and max. height of the tail) correlated with body size, but no additional effect of the habitat type was found. The analysis of microsatellites revealed a higher genetic diversity in forest ponds. However, no clear sign of inbreeding was observed in any agricultural population, suggesting some degree of gene flow between them. We conclude, that agricultural ponds can be suitable habitats for the Palmate Newt and that conservation effort should aim to preserve them. The observed effects on body size indicate the need to increase the quality of the aquatic and terrestrial habitat for early life stages of this newt species in agricultural landscapes.
This work addresses the challenge of calibrating multiple solid-state LIDAR systems. The study focuses on three different solid-state LIDAR sensors that implement different hardware designs, leading to distinct scanning patterns for each system. Consequently, detecting corresponding points between the point clouds generated by these LIDAR systems—as required for calibration—is a complex task. To overcome this challenge, this paper proposes a method that involves several steps. First, the measurement data are preprocessed to enhance its quality. Next, features are extracted from the acquired point clouds using the Fast Point Feature Histogram method, which categorizes important characteristics of the data. Finally, the extrinsic parameters are computed using the Fast Global Registration technique. The best set of parameters for the pipeline and the calibration success are evaluated using the normalized root mean square error. In a static real-world indoor scenario, a minimum root mean square error of 7 cm was achieved. Importantly, the paper demonstrates that the presented approach is suitable for online use, indicating its potential for real-time applications. By effectively calibrating the solid-state LIDAR systems and establishing point correspondences, this research contributes to the advancement of multi-LIDAR fusion and facilitates accurate perception and mapping in various fields such as autonomous driving, robotics, and environmental monitoring.
Potential impacts of invasive crayfish on native
benthic fish: shelter use and agonistic behaviour
(2023)
Spinycheek crayfish (Faxonius limosus) and signal crayfish (Pacifastacus leniusculus) are successful North American invasive crayfish species distributed throughout Europe. Both species compete with native benthic fish for shelter. In a laboratory approach, we assessed competition for shelter and antagonistic interactions between these invasive crayfish species and the native benthic fish species, stone loach (Barbatula barbatula) and bullhead (Cottus gobio). This allows for studying the potential impacts of invasive crayfish on native benthic fish. Spinycheek crayfish and signal crayfish were able to gain control of the shelter and could successfully displace both benthic fish species. For stone loach, the presence of crayfish significantly decreased their shelter use and caused several behavioural changes such as reduced activity and increased hiding behaviour outside the shelter. Although the shelter use by bullheads was not reduced, they displayed similar behavioural changes, if less intense. Invasive crayfish species showed remarkable combative interactions against both species of benthic fishes, evidenced by the high number of aggressive interactions, especially concerning stone loach. Our results highlight the pronounced dominance of invasive crayfish over benthic fish in terms of shelter competition and aggressive interactions under laboratory conditions, which consequently might promote the latter’s exposure to predation.
The Coronavirus Pandemic has influenced the lives of many people. We analyzed the effects of physical activity and stress on students’ motivation during the Pandemic. Participants were 254 university students who reported their academic motivation, physical activity, general stress, the Coronavirus Pandemic strain, and their Coronavirus stress. Women reported higher levels of Coronavirus stress, general stress, and motivation. The Coronavirus stress was predicted by the strain of the Coronavirus Pandemic but not by physical activity. General stress and gender predicted mastery goals, and performance goals were predicted by general stress. Physical activity was not related to students’ motivation during the Pandemic. Higher levels of general stress were associated with higher academic motivation. Negative emotions like stress could have enhanced students’ motivation during uncertain times of the Pandemic. Moreover, a moderate stress level could be favorable for academic dedication and achievement.
X-ray computer tomography (XRT) is a three-dimensional, nondestructive, and thus reproducible examination method that allows for the investigation of internal and external structures of objects. Due to its characteristics, the XRT technique has increasingly established itself as an alternative examination method and is also applied in the field of mineral processing. Within this work, XRT is used to investigate the influence of hydrochloric acid leaching of iron-rich bauxites on grain composition. Acid leaching is a promising method for the beneficiation of iron-rich bauxites for refractories. Many studies have already established that leaching with hydrochloric acid can reduce the Fe₂O₃ content in bauxites. However, apart from the influence of the leaching process on the composition of the bauxites, aspects such as the influence of the acid on the exact grain constitution or the porosity behavior have rarely been considered so far. To address these open questions, XRT analysis was used to examine and characterize various bauxites. By comparing identical grains before and after leaching, it was observed that in gibbsite bauxites the acid penetration is deeper, and the volume decreases significantly. In diasporic and boehmitic bauxites, clear leaching edges can be seen in which the iron content has been reduced.
Counts of SARS-CoV-2-related deaths have been key numbers for justifying severe political, social and economical measures imposed by authorities world-wide. A particular focus thereby was the concomitant excess mortality (EM), i.e. fatalities above the expected all-cause mortality (AM). Recent studies, inter alia by the WHO, estimated the SARS-CoV-2-related EM in Germany between 2020 and 2021 as high as 200 000. In this study, we attempt to scrutinize these numbers by putting them into the context of German AM since the year 2000. We propose two straightforward, age-cohort-dependent models to estimate German AM for the ‘Corona pandemic’ years, as well as the corresponding flu seasons, out of historic data. For Germany, we find overall negative EM of about −18 500 persons for the year 2020, and a minor positive EM of about 7000 for 2021, unveiling that officially reported EM counts are an exaggeration. In 2022, the EM count is about 41 200. Further, based on NAA-test-positive related death counts, we are able to estimate how many Germans have died due to rather than with CoViD-19; an analysis not provided by the appropriate authority, the RKI. Through 2020 and 2021 combined, our due estimate is at no more than 59 500. Varying NAA test strategies heavily obscured SARS-CoV-2-related EM, particularly within the second year of the proclaimed pandemic. We compensated changes in test strategies by assuming that age-cohort-specific NAA-conditional mortality rates during the first pandemic year reflected SARS-CoV-2-characteristic constants.
Men are currently underrepresented in traditionally female care-oriented (communal) engagement such as taking parental leave, whereas they are overrepresented in traditionally male (agentic) engagement such as breadwinning or leadership. We examined to what extent different prototypical representations of men affect men’s self-reported parental leave-taking intentions and more generally the future they can imagine for themselves with regard to work and care roles (i.e., their possible selves). We expected prototypes of men that combine the two basic stereotype dimensions of agency and communion to increase men’s communal intentions. In two experiments (N1 = 132, N2 = 233), we presented male participants with contrived newspaper articles that described the ideal man of today with varying degrees of agency and communion (between-subjects design with four conditions; combined agentic and communal vs. agentic vs. communal vs. control condition). Results of Experiment 1 were in line with the main hypothesis that especially presenting a combination of agency and communion increases men’s expectations for communal engagement: As compared to a control condition, men expected more to engage in caretaking in the future, reported higher parental leave-taking intentions, and tended to expect taking longer parental leave. Experiment 2 only partially replicated these findings, namely for parental leave-taking intentions. Both experiments additionally provided initial evidence for a contrast effect in that an exclusive focus on agency also increased men’s self-reported parental leave-taking intentions compared to the control condition. Yet, exclusively emphasizing communion in prototypes of men did not affect men’s communal intentions, which were high to begin with. We further did not find evidence for preregistered mechanisms. We discuss conditions and explanations for the emergence of these mixed effects as well as implications for the communication of gendered norms and barriers to men’s communal engagement more broadly.