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The loss of biodiversity is recognised on a global scale and also in the anthropogenic landscapes used for agriculture, now covering almost 50% of the global terrestrial land surface. In agriculture pesticides, biologically active chemicals are deliberately distributed to control pests, disease and weeds in the cropped areas. The quantification of remaining semi-naturals structures such as field margins and hedges is a prerequisite to understand the impact of pesticides on biodiversity, since these structures represent habitats for many organisms in agricultural landscapes. The presence of organisms in these habitats and crops is required to obtain an estimate of their potential pesticide exposure. In this text I provide studies on animal groups so far not addressed in risk assessment procedures for the regulation of pesticides such as amphibians, moths and bats. For all groups it becomes apparent that they are present in agricultural landscapes and potentially coincide with pesticide applications indicating a risk. Risk quantification also requires data on the sensitivity of organisms and here data for plants, amphibians and bees are presented. Effects translating to community level were studied for herbicide, insecticide and fertiliser effects in a natural system. After three years the treatments resulted in simplified plant communities with lower species numbers and a reduction in flowering plants. This reduction of flowers is used as an example for an indirect effect and was especially obvious for the effect of an herbicide on the common buttercup. Sublethal herbicide effects for a plant translated in an impact on feeding caterpillars, indicating a reduction in food quality. Insecticide inputs realistic for field margins also reduced moth pollination of white champion flowers by 30%. These indirect effects by distortions of food web characteristics are playing a critical role to understand declines in organism groups, however so far are not accounted for in pesticide risk assessment schemes. The current intense use of pesticides in agriculture and their inherent toxicity may lead to a chemical landscape fragmentation, where populations may not be connected anymore. Source-sink dynamics are important ecological processes and as a final result not only population size but also genetic population structure might be affected. Including potential pesticide impacts as costs in a model for amphibians migrating to breeding ponds in vineyards in Rhineland-Palatinate indicated the isolation of investigated populations. A first validation by analyzing the population structure of the European common frog confirmed the model prediction for some sites. For the regulation of pesticides in Europe a risk assessment is required and for the organisms of the terrestrial habitat a multitude of guidance documents is in place or is recently developed or improved. The results of the presented research indicate that wild plants and especially their reproductive flower stage are highly sensitive and risks are underestimated. Population recovery of arthropods needs a reevaluation at landscape scale and the addition of amphibian risk assessment in regulation procedures is suggested. However, developing or adopting risk assessment procedures and test systems is a time consuming task and therefore the establishment of risk management options is a pragmatic alternative with immediate effects. Artificial wetlands in the agricultural landscape proved to be important foraging sites for bats and their creation could mitigate negative pesticide effects. The integration of direct and indirect effects in a risk assessment scheme for all organism groups addressing also landscape scale and pesticide mixtures requires a long developing time. The establishment of model landscapes where management options and integrated pest management are applied on a larger scale would allow us to study pesticide effects in a realistic scenario and to develop an approach for the agriculture of the future.
In the last decades, it became evident that the world is facing an unprecedented, human-induced global biodiversity crisis with amphibians being one of the most threatened species groups. About 41% of the amphibian species are classified as endangered by the IUCN, but even in amphibian species that are listed as "least concern", population declines can be observed on a local level. With land-use change and agrochemicals (i.e. pesticides), two of the main drivers for this amphibian decline are directly linked to intensive agriculture, which is the dominant landscape type in large parts of Europe. Thus, understanding the situation of amphibians in the agricultural landscape is crucial for conservation measures. In the present thesis, I investigated the effects of viticulture on amphibian populations around Landau in der Pfalz (Germany) in terms of habitat use, pesticide exposure, biometric traits as well as genetic and age structure. From the perspective of amphibians, land-use change means usually the destruction of habitats in agricultural landscapes, which often leads to landscape fragmentation. Thus, I followed the question if also vineyards lead to the fragmentation of the landscape and if pesticides that are frequently used in viticulture have to be considered as a factor too, so if there is a chemical landscape fragmentation. Using telemetry, I could show that common toads (Bufo bufo) can be found directly in vineyards, but that they tend to avoid them as habitat. Analysing the genetic structure of common frogs (Rana temporaria) revealed that vineyards have to be considered as a barrier for amphibians. To identify if pesticides contribute to the resulting landscape fragmentation, I conducted an arena choice experiment in the laboratory in which I found evidence for an avoidance of pesticide-contaminated soil. Such an avoidance could be one of the underlying reasons for a potential chemical landscape fragmentation. By combining telemetry data with information about pesticide applications from local wine growers, I could show that a large part of the common toads is likely to come in contact with pesticides. Further, I demonstrated that the agricultural landscape, probably due to the application of pesticides, can have negative effects on the reproduction capacity of common toads. By studying palmate newts (Lissotriton helveticus) I found that adult newts from agricultural ponds are smaller than those from forest ponds. As I did not find differences in the age structure and growth, these differences might be carry-over effects from earlier life stages. While agricultural ponds might be suitable habitats for adult palmate newts, the potential carry-over effect indicates suboptimal conditions for larvae and/or juveniles. I conclude that the best management measure for sustaining amphibians in the agricultural landscape would be a heterogeneous cultural landscape with a mosaic of different habitat patches that work without or at least a reduced amount of pesticides. Green corridors between populations and different habitats would allow migrating individuals to avoid agricultural and thus pesticide-contaminated areas. This would reduce the pesticide exposure risk of amphibians, while preventing the fragmentation of the landscape and thus the isolation of populations.
With 47% land coverage in 2016, agricultural land was one of the largest terrestrial biomes in Germany. About 70% of the agricultural land was cropped area with associated pesticide applications. Agricultural land also represents an essential habitat for amphibians. Therefore, exposure of amphibians to agrochemicals, such as fertilizers and pesticides, seems likely. Pesticides can be highly toxic for amphibians, even a fraction of the original application rate may result in high amphibian mortality.
To evaluate the potential risk of pesticide exposure for amphibians, the temporal coincidence of amphibian presence on agricultural land and pesticide applications (N = 331) was analyzed for the fire-bellied toad (Bombina bombina), moor frog (Rana arvalis), spadefoot toad (Pelobates fuscus) and crested newt (Triturus cristatus) during spring migration. In 2007 and 2008, up to 80% of the migrating amphibians temporally coincided with pesticide applications in the study area of Müncheberg, about 50 km east of Berlin. Pesticide interception by plants ranged between 50 to 90% in winter cereals and 80 to 90% in winter rape. The highest coincidence was observed for the spadefoot toad, where 86.6% of the reproducing population was affected by a single pesticide in winter rape during stem elongation with 80% pesticide interception by plants. Late migrating species, such as the fire-bellied toad and the spadefoot toad, overlapped more with pesticide applications than early migrating species, such as the moor frog, did. Under favorable circumstances, the majority of early migrants may not coincide with the pesticide applications of arable fields during spring migration.
To evaluate the potential effect of pesticide applications on populations of the common frog (Rana temporaria), a landscape genetic study was conducted in the vinicultural area of Southern Palatinate. Due to small sample sizes at breeding sites within viniculture, several DNA sampling methods were tested. Furthermore, the novel repeated randomized selection of genotypes approach was developed to utilize genetic data from siblings for more reliable estimates of genetic parameters. Genetic analyses highlighted three of the breeding site populations located in viniculture as isolated from the meta-population. Genetic differentiation among breeding site populations in the viniculture (median pairwise FST=0.0215 at 2.34 km to 0.0987 at 2.39 km distance) was higher compared to genetic differentiation among breeding site populations in the Palatinate Forest (median pairwise FST=0.0041 at 5.39 km to 0.0159 at 9.40 km distance).
The presented studies add valuable information about the risk of pesticide exposure for amphibians in the terrestrial life stage and possible effects of agricultural land on amphibian meta-populations. To conserve endemic amphibian species and their (genetic) diversity in the long run, the risk assessment of pesticides and applied agricultural management measures need to be adjusted to protect amphibians adequately. In addition, other conservation measures such as the creation of new suitable breeding site should be considered to improve connectivity between breeding site populations and ensure the persistence of amphibians in the agricultural land.
The largest population of the anadromous Allis shad (A. alosa) of the 19th century was found in River Rhine and has to be considered extinct today. To facilitate the return of A. alosa into River Rhine an EU LIFE-project was initiated in 2007. The overall objective of this thesis was to assist aquaculture and stocking-measures at River Rhine, as well as to support restoration and conservation of populations of Allis shad in Europe.
By culturing the free-swimming nematode T. aceti in a solution of cider vinegar we developed a cost-effective live food organism for the larviculture of fish. As indicated by experiments with C. maraena, T. aceti cannot be regarded as an alternative to Artemia nauplii. However it has to be considered a suitable supplemental feed in the early rearing of C. maraena by providing essential fatty acids, thereby optimizing growth.
Also mass-marking practices with Oxytetracycline, as they are applied in the restocking of Allis shad have been evaluated. In experiments with D. rerio we demonstrated that water hardness can detrimentally affect mortality during marking and has to be considered crucial in the development of marking protocols for freshwater fish.
In order to get independent from wild spawners an ex-situ Broodstock-facility for Allis shad was established in 2011. Upon examination of two complete year classes of this broodstock, we found a high prevalence of various malformations, which could be traced back to distinct cysts developing one month post hatch. Despite applying a variety of clinical tests we could not identify any infectious agents causing these malformations. The observed malformations are probably a consequence of suboptimal feeding practices or the properties of the physio-chemical rearing environment.
The decline of stocks of A. alosa in Europe has been largely explained with the increase of river temperatures as a consequence of global warming. By investigating the temperature physiology of larval Allis shad we demonstrated that A. alosa ranges among the most thermo-tolerant species in Europe and that correlations between rising temperatures and the disappearance of this species have to be understood in a synecological context and by integrating a variety of stressors other than temperature. By capturing and examining juvenile and adult Allis shad from River Rhine, we demonstrated the first natural reproduction of A. alosa in River Rhine since nearly 100 years and the success of stocking measures within the framework of the LIFE project.
Agricultural plastic covers made from polyethylene (PE) and polypropylene (PP) provide increased yields and an improved crop quality. However, such covers are suspected of partially breaking down into smaller debris and thereby contributing to soil pollution with microplastics. To scrutinize this, we randomly sampled 240 topsoil cores (0–5 cm) from eight fields which were covered with fleeces, perforated foils, and plastic mulches for less than 2 years. Samples from the field periphery (50 m perimeter) served as a reference. Visual plastic debris > 2 mm was analyzed by Fourier transform infrared spectroscopy. Smaller, soil-associated plastic debris was dispersed from 50 g of fine soil (≤ 2 mm) using sodium hexametaphosphate solution and density-separated with saturated NaCl solution. The collected PE, PP, and polystyrene (PS) debris was selectively dissolved in a mixture of 1,2,4-trichlorobenzene and p-xylene at 150 °C and quantified by pyrolysis–gas chromatography–mass spectrometry (Py-GC/MS). We counted six PE and PS fragments > 2 mm in two out of eight fields. By contrast, Py-GC/MS detected PE, PP, and PS contents in the fine soil of six fields (6 % of all samples). In three fields, PE levels of 3–35 mg kg−1 were potentially associated with the use of thinner and less durable perforated foils (40 µm thickness). This was slightly more pronounced at field edges where the plastic covers are turned and weighed down. By contrast, 50 µm thick PE films were not shown to emit any plastic debris. PP contents of 5–10 mg kg−1 were restricted to single observations in the field centers of three sites. At one site, we found expanded PS particles > 2 mm that concurred with elevated PS levels (8–19 mg kg−1) in the fine soil. Both PP and PS were distributed indistinctly across sites so that their source remained unresolved. In addition, the extent to which plastic contents of up to 7 mg kg−1 in the field periphery of some sites were attributed to wind drift from the covered fields or from external sources needs to be investigated in future studies. Our results suggest that the short-term use of thicker and more durable plastic covers should be preferred over thinner or perforated films to limit plastic emissions and accumulation in soil.
The growing numbers of breeding rooks (Corvus frugilegus) in the city of Landau (Rhineland- Palatinate, Germany) increase the potential for conflict between rooks and humans, which is mainly associated with noise and faeces. Therefore, the aim of this work is a better understanding of the breeding tree selection of the rook in order to develop options for action and management in the future.
Part I of this thesis provides general background information on the rook and includes mapping of the rookeries in the Anterior Palatinate and South Palatinate including Landau in the year 2020. That mapping revealed that the number of rural colonies has decreased, while the number of urban colonies has increased in the study area in the last few years. In line with current literature, tree species and tree size were important criteria for breeding tree selection. However, the mapping showed that additional factors must be important as well.
Therefore, as rooks seem to often breed along traffic axes, Part II of this thesis examines how temperature, artificial light and noise, which are all linked to traffic axes, affect the breeding tree selection of the rook in the city of Landau. The following three hypotheses are developed: (1) manually selected breeding trees (Bm) have a warmer microclimate than manually selected non-breeding trees (Nm) or randomly selected non-breeding trees (Nr), (2) Bm are exposed to a higher light level than Nm or Nr and (3) Bm are exposed to a higher noise level than Nm or Nr. To test these hypotheses, 15 Bm, 13 Nm and 16 Nr are investigated.
The results show that Bm were exposed to more noise than both types of non-breeding trees (μBm, noise = 36.52481 dB, μNm, noise = 31.27229 dB, μNr, noise = 29.17417 dB) where the difference between Bm and Nr was significant. In addition, there was a tendency for Bm to be exposed to less light (μBm, light = 0.356 lx) than Nm (μNm, light = 0.4107692 lx) and significantly less light than Nr (μNr, light = 1.995 lx), while temperature did not differ between the groups (μBm, temp = 16.90549 °C, μNm, temp = 16.93118 °C, μNr, temp = 17.28639 °C).
This study shows for the first time that rooks prefer trees which are exposed to low light levels and high noise levels, i.e. more intense traffic noise, for breeding. It can only be speculated that the cause of this is lower enemy pressure at such sites. The fact that temperature does not seem to have any influence on breeding tree selection may be due to only small temperature differences at nest height, which might be compensated by breeding behaviour. Consequently, in the long term one management approach could be to divert traffic from inner-city areas, especially schools and hospitals, to bypasses. If tree genera suitable for rooks, such as plane trees, are planted along the bypasses, those sites could provide suitable alternative habitats to inner-city breeding locations, which become less attractive for breeding due to noise reduction. In the short term in addition to locally implemented repellent measures the most effective approach is to strengthen rook acceptance among the population. However, further research is needed to verify the results of this thesis and to gain further insights into rook breeding site selection in order to develop effective management measures.
The European weatherfish (Misgurnus fossilis) is a benthic freshwater fish species belonging to the family Cobitidae, that is subjected to a considerable decline in many regions across its original distribution range. Due to its cryptic behavior and low economic value, the causes of threat to weatherfish remained partly unknown and the species is rarely at the center of conservation efforts. In order to address these concerns, the overall aim of the present thesis was to provide a comprehensive approach for weatherfish conservation, including the development of stocking measures, investigations on the species autecology and the evaluation of potential threats. The first objective was to devise and implement a regional reintroduction and stock enhancement program with hatchery-reared weatherfish in Germany. Within this program (2014-2016), a total number of 168,500 juvenile weatherfish were stocked to seven water systems. Recaptures of 45 individuals at two reintroduction sites supported the conclusion that the developed stocking strategy was appropriate. In order to broaden the knowledge about weatherfish autecology and thereby refining the rearing conditions and the selection of appropriate stocking waters, the second objective was to investigate the thermal requirements of weatherfish larvae. Here, the obtained results revealed that temperatures higher than previously suggested were tolerated by larvae, whereas low temperatures within the range of likely habitat conditions increased mortality rates. As weatherfish can be frequently found in agriculturally impacted waters (e.g. ditch systems), they are assumed to have an increased probability to be exposed to chemical stress. Since the resulting risk has not yet been investigated with a focus on weatherfish, the third objective was to provide a methodical foundation for toxicity testing that additionally complies with the requirements of alternative test methods. For this purpose, the acute fish embryo toxicity test was successfully transferred to weatherfish and first results exhibited that sensitivity of weatherfish towards a tested reference substance (3,4-dichloroaniline) was highest compared to other species. On the basis of these findings, the fourth objective was to apply weatherfish embryos for multiple sediment bioassays in order to investigate teratogenic effects derived from sediment-associated contaminants. In this context, weatherfish revealed particular sensitivity to water extractable substances, indicating that sediment contamination might pose a considerable risk. Moreover, as an endangered benthic fish species with high ecological relevance for European waters that are specifically exposed to hazardous contaminants, the weatherfish might be a prospective species for an ecological risk assessment of sediment toxicity. Overall, the present thesis contributed to the conservation of weatherfish by considering a variety of aspects that interact and reinforce one another in order to achieve improvements for the species situation.
In dieser Studie sollte hinsichtlich der Entwicklung eines faunistisch begründeten Monitoringkonzeptes für hydrologische Interaktionen untersucht werden, inwieweit die Meiofauna in Hyporheal und Grundwasser in der Lage ist, Oberflächenwasser-Grundwasser-Interaktionen zu reflektieren und die hydrologischen Verhältnisse einer Uferfiltrationsanlage aufzuzeigen. Dafür wurden hydrochemische und faunistische Analysen bezüglich ihrer Aussagekraft miteinander verglichen. Außerdem wurde die Anwendbarkeit des Grundwasser-Fauna-Index (GFI) zur Beurteilung der Stärke des Oberflächenwassereinflusses auf unterirdisches Wasser überprüft. Für die Untersuchung wurde die Uferfiltrationsanlage Flehe der Stadtwerke Düsseldorf ausgewählt, auf deren Gelände 70 vertikale Entnahmebrunnen über eine Strecke von 1,4 km eine Brunnengalerie bilden, die im Jahr insgesamt 11 Mio. m3 Wasser fördert. Am Untersuchungsstandort wurden insgesamt 15 Observationspegel von Februar 2005 bis Januar 2006 faunistisch und hydrochemisch beprobt. Die physikochemische Untersuchung ergab eine Aufteilung des Untersuchungsgebietes in vier Zonen. Neben dem Oberflächenwasser des Rheins [OW Rhein] konnten das Uferfiltrationswasser zwischen Rhein und den Entnahmebrunnen [Hyporheal], alluviales Grundwasser hinter der Brunnengalerie [Alluv. GW] und bei größerer Entfernung zum Rhein landseitiges Grundwasser [landseitiges GW] differenziert werden. Dabei wies der Rhein die typische Charakteristik eines Fließgewässers auf, gekennzeichnet durch eine große Temperaturamplitude mit einem Sommermaximum, hohen Sauerstoffkonzentrationen mit einem Wintermaximum und erhöhten AOX-, DOC- und SAK254nm-Werten. Am Uferfiltrat der Gruppe [Hyporheal] wurde die Wirksamkeit der mikrobiellen Abbauprozesse während der Uferfiltration mit deutlichen Reduzierungen der DOC- und SAK- und Sauerstoffkonzentrationen ersichtlich. Die Gruppe [Alluv. GW] und verstärkt das [landseitige GW] waren durch eine höhere elektrische Leitfähigkeit und erhöhten Bor- und Sulfatkonzentrationen sowie z. T. geringen Sauerstoffgehalten gekennzeichnet. Anhand dieser hydrochemischen Differenzen konnte die unterschiedliche Herkunft des Wassers im Untersuchungsgebiet ermittelt werden Die faunistischen Untersuchungen zeigten ein differenzierteres Bild von den hydrologischen Verhältnissen als die Hydrochemie. Insgesamt konnten fünf ökologische Gruppen ermittelt werden. Die Gruppe [Hyporheal 1] der A-Pegelreihe wies als einzige euryöke Arten auf, die einen stärkeren Oberflächenwassereinfluss anzeigten. Im [Hyporheal 2] der B-Pegelreihe konnte eine artenreiche ökotonale Fauna nachgewiesen werden, während die Proben des alluvialen Grundwassers der C-Pegelreihe [Alluv. GW (Pumpe)] den Einfluss der Entnahmebrunnen reflektierten. In den Pegeln des alluvialen Grundwassers [Alluv. GW] wurde aufgrund der geringeren Beeinflussung der Pumpen artenreichere Zoozönosen vorgefunden. Das [landseitige GW] wies schließlich eine arten- und abundanzarme Fauna auf, die charakteristisch ist für Grundwasser mit geringem Oberflächenwassereinfluss. Folglich konnte die Fauna nicht nur die Herkunft des Grundwassers anzeigen, sondern auch den Einfluss und die Intensität des Oberflächenwassers auf das Grundwasser sowie anthropogene Störungen wie eine verstärkte Kolmation bzw. die Effekte der Wasserentnahme durch die Brunnen. Die Meiofauna im Grundwasserleiter der Uferfiltrationsanlage dürfte somit als Indikator für hydrologische Verhältnisse gut geeignet sein. Der Grundwasser-Fauna-Index konnte als der wichtigste Faktor aller physikochemischen Parameter ermittelt werden, der die faunistischen Gruppen am stärksten beeinflusst. Außerdem war der GFI in der Lage, die Auswirkung und Intensität des Einflusses von Oberflächenwasser auf die einzelnen Probestandorte anzuzeigen. Die hier dargestellten Ergebnisse zeigen, dass die Grundwasserfauna und der GFI viel versprechende Methoden zur Bewertung der hydrologischen Verhältnisse, insbesondere des Einflusses von Oberflächenwasser auf das Grundwasser sind. Daher sind sie als Ergänzung zur hydrochemischen Analyse zu empfehlen und können auch einen wichtigen Beitrag für die Vulnerabilitätsabschätzung leisten.
Während es eine Vielzahl von Arbeiten zu der technologischen Entwicklung im Bereich der erneuerbaren Energien gibt, fehlt es jedoch bislang an einer mikroökonomischen Analyse
der Verhaltensmuster der Akteure im Umfeld von Anlagen nach dem EEG. Als Akteure kommen hier in erster Linie der Anlagenbetreiber selbst und der Staat in Betracht.
Im Hinblick auf Anlagenbetrieb und Vergütung der erzeugten Energie können beide mit unterschiedlichsten Interessen und Nutzenkalkülen aufeinander treffen. Diese Arbeit untersucht
mikroökonomische Aspekte des EEG-Förderungssystems. Im Mittelpunkt der Betrachtung stehen die Förderungsmechanismen für Biogasanlagen, die im Hinblick auf mögliche Prinzipal-Agenten-Konflikte einer Untersuchung unterzogen werden.
Systemic neonicotinoids are one of the most widely used insecticide classes worldwide. In addition to their use in agriculture, they are increasingly applied on forest trees as a protective measure against insect pests. However, senescent leaves containing neonicotinoids might, inter alia during autumn leaf fall, enter nearby streams. There, the hydrophilic neonicotinoids may be remobilized from leaves to water resulting in waterborne exposure of aquatic non-target organisms. Despite the insensitivity of the standard test species Daphnia magna (Crustacea, Cladocera) toward neonicotinoids, a potential risk for aquatic organisms is evident as many other aquatic invertebrates (in particular insects and amphipods) display adverse effects when exposed to neonicotinoids in the ng/L- to low µg/L-range. In addition to waterborne exposure, in particular leaf-shredding invertebrates (= shredders) might be adversely affected by the introduction of neonicotinoid-contaminated leaves into the aquatic environment since they heavily rely on leaf litter as food source. However, dietary neonicotinoid exposure of aquatic shredders has hardly received any attention from researchers and is not considered during aquatic environmental risk assessment. The primary aim of this thesis is, therefore, (1) to characterize foliar neonicotinoid residues and exposure pathways relevant for aquatic shredders, (2) to investigate ecotoxicological effects of waterborne and dietary exposure on two model shredders, namely Gammarus fossarum (Crustacea, Amphipoda) and Chaetopteryx villosa (Insecta, Trichoptera), and (3) to identify biotic and abiotic factors potentially modulating exposure under field conditions.
During the course of this thesis, ecotoxicologically relevant foliar residues of the neonicotinoids imidacloprid, thiacloprid and acetamiprid were quantified in black alder trees treated at field relevant levels. A worst-case model – developed to simulate imidacloprid water concentrations resulting from an input of contaminated leaves into a stream – predicted only low aqueous imidacloprid concentrations (i.e., ng/L-range). However, the model identified dietary uptake as an additional exposure pathway relevant for shredders up to a few days after the leaves’ introduction into the stream. When test organisms were simultaneously exposed (= combined exposure) to neonicotinoids leaching from leaves into the water and via the consumption of contaminated leaves, adverse effects exceeded those observed under waterborne exposure alone. When exposure pathways were separated using a flow-through system, dietary exposure towards thiacloprid-contaminated leaves caused similar sublethal adverse effects in G. fossarum as observed under waterborne exposure. Moreover, the effect sizes observed under combined exposure were largely predictable using the reference model “independent action”, which assumes different molecular target sites to be affected. Dietary toxicity for shredders might, however, be reduced under field conditions since UV-induced photodegradation and leaching decreased imidacloprid residues in leaves and thereby the toxicity for G. fossarum. In contrast, both shredders were found unable to actively avoid dietary exposure. This thesis thus recommends considering dietary exposure towards systemic insecticides, such as neonicotinoids, already during their registration to safeguard aquatic shredders, associated ecosystem functions (e.g., leaf litter breakdown) and ultimately ecosystem integrity.