Doctoral Thesis
Refine
Year of publication
- 2017 (32) (remove)
Document Type
- Doctoral Thesis (32) (remove)
Keywords
- AD(H)S Bindung (1)
- ADHS (1)
- ADS (1)
- Analoge Methoden (1)
- Aquatic Guidance Document (1)
- Bestäubung (1)
- Betriebspädagogik (1)
- Bill Clinton (1)
- Biodiversität (1)
- Empirische Forschung (1)
- Environmental organic chemistry (1)
- Fachhochschule (1)
- Führung (1)
- Führungskultur (1)
- GIS (1)
- Geothermie (1)
- Governance (1)
- Grundwasser (1)
- Grundwasserfauna (1)
- Handlungstheorie (1)
- Hochschulorganisation (1)
- Holzhaus (1)
- ISO 9001 (1)
- Irak Krieg (1)
- Kant, Immanuel (1)
- Klimaerwärmung (1)
- Konflikt (1)
- Konfliktfähigkeit (1)
- Konfliktkultur (1)
- Leiblichkeit (1)
- Lernerorientierte Qualitätstestierung (LQW) (1)
- Marketing (1)
- Mentalität (1)
- Micropollutants (1)
- Nato (1)
- Nato Globale Partner (1)
- Nato Response Force (1)
- Nato Transformation (1)
- Natürliche Schädlingskontrolle (1)
- Neokonservative (1)
- Nährstoffverfügbarkeit (1)
- Obama (1)
- Offensiver Neorealismus (1)
- Organisationsentwicklung (1)
- Pflege (1)
- Pflegedienst (1)
- Preemptive strike (1)
- Professionalität (1)
- Qualitätsmanagement (1)
- Qualitätssicherung (1)
- Revolution in military affairs (1)
- Rheingau-Taunus-Kreis (1)
- Sicherheit (1)
- Spielarten des Kapitalismus (1)
- Standortanalyse (1)
- Staubewässerung (1)
- Systemische Beratung (1)
- Temperaturpräferenzen (1)
- Transformation products (1)
- Unternehmensflexibilität (1)
- Varieties of Capitalism (1)
- Very High Readiness Joint Task Foce (1)
- Wastewater (1)
- Weiterbildung (1)
- agroecosystems (1)
- anthropogener Wärmeeintrag (1)
- aquatic invertebrates (1)
- aquatic macrophytes (1)
- assertive multilateralism (1)
- dialogische Führung (1)
- ecosystem services (1)
- grassland irrigation (1)
- landscape complexity (1)
- offensive neorealism (1)
- pesticide risk assessment (1)
- stream mesocosm (1)
- Österreich (1)
Institute
- Fachbereich 7 (9)
- Fachbereich 5 (4)
- Institut für Integrierte Naturwissenschaften, Abt. Chemie (4)
- Institut für Umweltwissenschaften (4)
- Institut für Erziehungswissenschaft (3)
- Institut für Sozialwissenschaften (2)
- Fachbereich 6 (1)
- Institut für Informatik (1)
- Institut für Integrierte Naturwissenschaften, Abt. Biologie (1)
- Institut für Integrierte Naturwissenschaften, Abt. Geographie (1)
Die Forschung im Bereich der modellbasierten Objekterkennung und Objektlokalisierung hat eine vielversprechende Zukunft, insbesondere die Gebäudeerkennung bietet vielfaltige Anwendungsmöglichkeiten. Die Bestimmung der Position und der Orientierung des Beobachters relativ zu einem Gebäude ist ein zentraler Bestandteil der Gebäudeerkennung.
Kern dieser Arbeit ist es, ein System zur modellbasierten Poseschätzung zu entwickeln, das unabhängig von der Anwendungsdomäne agiert. Als Anwendungsdomäne wird die modellbasierte Poseschätzung bei Gebäudeaufnahmen gewählt. Vorbereitend für die Poseschätzung bei Gebäudeaufnahmen wird die modellbasierte Erkennung von Dominosteinen und Pokerkarten realisiert. Eine anwendungsunabhängige Kontrollstrategie interpretiert anwendungsspezifische Modelle, um diese im Bild sowohl zu lokalisieren als auch die Pose mit Hilfe dieser Modelle zu bestimmen. Es wird explizit repräsentiertes Modellwissen verwendet, sodass Modellbestandteilen Bildmerkmale zugeordnet werden können. Diese Korrespondenzen ermöglichen die Kamerapose aus einer monokularen Aufnahme zurückzugewinnen. Das Verfahren ist unabhängig vom Anwendungsfall und kann auch mit Modellen anderer rigider Objekte umgehen, falls diese der definierten Modellrepräsentation entsprechen. Die Bestimmung der Pose eines Modells aus einem einzigen Bild, das Störungen und Verdeckungen aufweisen kann, erfordert einen systematischen Vergleich des Modells mit Bilddaten. Quantitative und qualitative Evaluationen belegen die Genauigkeit der bestimmten Gebäudeposen.
In dieser Arbeit wird zudem ein halbautomatisches Verfahren zur Generierung eines Gebäudemodells vorgestellt. Das verwendete Gebäudemodell, das sowohl semantisches als auch geometrisches Wissen beinhaltet, den Aufgaben der Objekterkennung und Poseschätzung genügt und sich dennoch an den bestehenden Normen orientiert, ist Voraussetzung für das Poseschätzverfahren. Leitgedanke der Repräsentationsform des Modells ist, dass sie für Menschen interpretierbar bleibt. Es wurde ein halbautomatischer Ansatz gewählt, da die automatische Umsetzung dieses Verfahrens schwer die nötige Präzision erzielen kann. Das entwickelte Verfahren erreicht zum einen die nötige Präzision zur Poseschätzung und reduziert zum anderen die Nutzerinteraktionen auf ein Minimum. Eine qualitative Evaluation belegt die erzielte Präzision bei der Generierung des Gebäudemodells.
Agricultural land-use may lead to brief pulse exposures of pesticides in edge-of-field streams, potentially resulting in adverse effects on aquatic macrophytes, invertebrates and ecosystem functions. The higher tier risk assessment is mainly based on pond mesocosms which are not designed to mimic stream-typical conditions. Relatively little is known on exposure and effect assessment using stream mesocosms.
Thus the present thesis evaluates the appliacability of the stream mesocosms to mimic stream-typical pulse exposures, to assess resulting effects on flora and fauna and to evaluate aquatic-terrestrial food web coupling. The first objective was to mimic stream-typical pulse exposure scenarios with different durations (≤ 1 to ≥ 24 hours). These exposure scenarios established using a fluorescence tracer were the methodological basis for the effect assessment of an herbicide and an insecticide. In order to evaluate the applicability of stream mesocosms for regulatory purposes, the second objective was to assess effects on two aquatic macrophytes following a 24-h pulse exposure with the herbicide iofensulfuron-sodium (1, 3, 10 and 30 µg/L; n = 3). Growth inhibition of up to 66 and 45% was observed for the total shoot length of Myriophyllum spicatum and Elodea canadensis, respectively. Recovery of this endpoint could be demonstrated within 42 days for both macrophytes. The third objective was to assess effects on structural and functional endpoints following a 6-h pulse exposure of the pyrethroid ether etofenprox (0.05, 0.5 and 5 µg/L; n = 4). The most sensitive structural (abundance of Cloeon simile) and functional (feeding rates of Asellus aquaticus) endpoint revealed significant effects at 0.05 µg/L etofenprox. This concentration was below field-measured etofenprox concentrations and thus suggests that pulse exposures adversely affect invertebrate populations and ecosystem functions in streams. Such pollutions of streams may also result in decreased emergence of aquatic insects and potentially lead to an insect-mediated transfer of pollutants to adjacent food webs. Test systems capable to assess aquatic-terrestrial effects are not yet integrated in mesocosm approaches but might be of interest for substances with bioaccumulation potential. Here, the fourth part provides an aquatic-terrestrial model ecosystem capable to assess cross-ecosystem effects. Information on the riparian food web such as the contribution of aquatic (up to 71%) and terrestrial (up to 29%) insect prey to the diet of the riparian spider Tetragnatha extensa was assessed via stable isotope ratios (δ13C and δ15N). Thus, the present thesis provides the methodological basis to assess aquatic-terrestrial pollutant transfer and effects on the riparian food web.
Overall the results of this thesis indicate, that stream mesocosms can be used to mimic stream-typical pulse exposures of pesticides, to assess resulting effects on macrophytes and invertebrates within prospective environmental risk assessment (ERA) and to evaluate changes in riparian food webs.
Pelagic oxyclines, the transition zone between oxygen rich surface waters and oxygen depleted deep waters, are a common characteristic of eutrophic lakes during summer stratification. They can have tremendous effects on the biodiversity and the ecosystem functioning of lakes and, to add insult to injury, are expected to become more frequent and more pronounced as climate warming progresses. On these grounds, this thesis endeavors to advance the understanding of formation, persistence, and consequences of pelagic oxyclines: We test, whether the formation of metalimnetic oxygen minima is intrinsically tied to a locally enhanced oxygen consuming process, investigate the relative importance of vertical physical oxygen transport and biochemical oxygen consumption for the persistence of pelagic oxyclines, and finally assess their potential consequences for whole lake cycling. To pursue these objectives, the present thesis nearly exclusively resorts to in situ measurements. Field campaigns were conducted at three lakes in Germany featuring different types of oxyclines and resolved either a short (hours to days) or a long (weeks to months) time scale. Measurements comprised temperature, current velocity, and concentrations of oxygen and reduced substances in high temporal and vertical resolution. Additionally, vertical transport was estimated by applying the eddy correlation technique within the pelagic region for the first time. The thesis revealed, that the formation of metalimnetic oxygen minima does not necessarily depend on locally enhanced oxygen depletion, but can solely result from gradients and curvatures of oxygen concentration and depletion and their relative position to each other. Physical oxygen transport was found to be relevant for oxycline persistence when it considerably postponed anoxia on a long time scale. However, its influence on oxygen dynamics was minor on short time scales, although mixing and transport were highly variable. Biochemical consumption always dominated the fate of oxygen in pelagic oxyclines. It was primarily determined by the oxidative breakdown of organic matter originating from the epilimnion, whereas in meromictic lakes, the oxidation of reduced substances dominated. Beyond that, the results of the thesis emphasize that pelagic oxyclines can be a hotspot of mineralization and, hence, short-circuit carbon and nutrient cycling in the upper part of the water column. Overall, the present thesis highlights the importance of considering physical transport as well as biochemical cycling in future studies.
World’s ecosystems are under great pressure satisfying anthropogenic demands, with freshwaters being of central importance. The Millennium Ecosystem Assessment has identified anthropogenic land use and associated stressors as main drivers in jeopardizing stream ecosystem functions and the
biodiversity supported by freshwaters. Adverse effects on the biodiversity of freshwater organisms, such as macroinvertebrates, may propagate to fundamental ecosystem functions, such as organic matter breakdown (OMB) with potentially severe consequences for ecosystem services. In order to adequately protect and preserve freshwater ecosystems, investigations regarding potential and observed as well as direct and indirect effects of anthropogenic land use and associated stressors (e.g. nutrients, pesticides or heavy metals) on ecosystem functioning and stream biodiversity are needed. While greater species diversity most likely benefits ecosystem functions, the direction and magnitude of changes in ecosystem functioning depends primarily on species functional traits. In this context, the functional diversity of stream organisms has been suggested to be a more suitable predictor of changes in ecosystem functions than taxonomic diversity.
The thesis aims at investigating effects of anthropogenic land use on (i) three ecosystem functions by anthropogenic toxicants to identify effect thresholds (chapter 2), (ii) the organic matter breakdown by three land use categories to identify effects on the functional level (chapter 3) and (iii)on the stream community along an established land-use gradient to identify effects on the community level.
In chapter 2, I reviewed the literature regarding pesticide and heavy metal effects on OMB, primary production and community respiration. From each reviewed study that met inclusion criteria, the toxicant concentration resulting in a reduction of at least 20% in an ecosystem function was standardized based on laboratory toxicity data. Effect thresholds were based on the relationship between ecosystem functions and standardized concentration-effect relationships. The analysis revealed that more than one third of pesticide observations indicated reductions in ecosystem functions at concentrations that are assumed being protective in regulation. However, high variation within and between studies hampered the derivation of a concentration-effect relationship and thus effect thresholds.
In chapter 3, I conducted a field study to determine the microbial and invertebrate-mediated OMB by deploying fine and coarse mesh leaf bags in streams with forested, agricultural, vinicultural
and urban riparian land use. Additionally, physicochemical, geographical and habitat parameters were monitored to explain potential differences in OMB among land use types and sites. Regarding results, only microbial OMB differed between land use types. The microbial OMB showed a negative relationship with pH while the invertebrate-mediated OMB was positively related to tree cover. OMB responded to stressor gradients rather than directly to land use.
In chapter 4, macroinvertebrates were sampled in concert with leaf bag deployment and after species identification (i) the taxonomic diversity in terms of Simpson diversity and total taxonomic
richness (TTR) and (ii) the functional diversity in terms of bio-ecological traits and Rao’s quadratic entropy was determined for each community. Additionally, a land-use gradient was established and the response of the taxonomic and functional diversity of invertebrate communities along this gradient was investigated to examine whether these two metrics of biodiversity are predictive for the rate of OMB. Neither bio-ecological traits nor the functional diversity showed a significant relationship with
OMB. Although, TTR decreased with increasing anthropogenic stress and also the community structure and 26 % of bio-ecological traits were significantly related to the stress gradient, any of these shifts propagated to OMB.
Our results show that the complexity of real-world situations in freshwater ecosystems impedes the effect assessment of chemicals and land use for functional endpoints, and consequently our potential to predict changes. We conclude that current safety factors used in chemical risk assessment may not be sufficient for pesticides to protect functional endpoints. Furthermore, simplifying real-world stressor gradients into few land use categories was unsuitable to predict and quantify losses in OMB. Thus, the monitoring of specific stressors may be more relevant than crude land use categories to detect effects on ecosystem functions. This may, however, limit the large scale assessment of the status of OMB. Finally, despite several functional changes in the communities the functional diversity over several trait modalities remained similar. Neither taxonomic nor functional diversity were suitable predictors of OMB. Thus, when understanding anthropogenic impacts on the linkage between biodiversity and ecosystem functioning is of main interest, focusing on diversity metrics that are clearly linked to the stressor in question (Jackson et al. 2016) or integrating taxonomic and functional metrics (Mondy et al., 2012) might enhance our predictive capacity.
Gefährdete Weltmacht USA
(2017)
Aims and findings of the dissertation
The completed research uses holistic, politological and historical approaches to present how, during the studied period of the administrations of Clinton to Obama, the liberal, rule-based world order system is gradually supplemented and replaced by a system of realist imposition of vital interests that have short-term effects, preferring military means combined with continuous military optimisation. This also explains a continuity between the leading-power policy of administrations in this study (1993-2017) and the subsequent period of the “transactional leadership of Trump”(1), with its recognizable, far-reaching effects of aiming to reduce idealistic Grand Strategy elements and measures of a benevolent order by passing on costs to and reducing the benefits of European NATO allies. The results of this dissertation, such as the increasingly evident dissolution of a multilateral fundamental order, therefore indicate that Trump’s foreign and security policy to date should be regarded as a clearly noticeable crisis symptom, rather than the cause of a decline in the world order established after 1945. This decline is synonymous with the erosion of the transatlantically initiated bipolar “American system”. Its implementation was the result of the “lesson of two world wars”, based on modern concepts of order introduced by the Enlightenment and the founding criteria of the United States: thus its dissolution is also an indicator of the failure of contemporary criteria of order that thrive in the “American way of life”.
The cause of the described development is shown to be a constantly exacerbating overall threat, from Clinton to Obama, which is connected to the consistent erosion of US supremacy. Among other aspects, this is based on climate change effects postulated in 1979, which multiply the threat while coinciding with American peak production of fossil fuels and increased demand on resources in the context of dwindling raw material resources. Furthermore, during the period of this study, the “US conservative revolution”, which began in the 1980s, increasingly affected foreign and security policy, combining with a consolidation in the influence of corporations and lobby groups in fields such as policy implementation and new underlying conditions. They include the onset of digitisation, entailing a high consumption of resources, and a growing world population faced with specific demographic indicators. Additionally, the maintenance of the armaments sector, originally a result of bipolar development, as the economic basis of military supremacy and the slow decline of the Dollar hegemony since around 1973, should also be taken into account. Complex interaction between Grand Strategy implementation according to the premise of expanding US-American dominance under neoconservative and Christian Right-wing influences, as well as asymmetrical and reactivated conventional security threats and threat multipliers clearly indicate the linear development of the overall threat in the period between 1993 and 2017: in the context of Grand Strategy statements, above all the understanding of defence against this threat, of the latter’s multiplying factors and the market economy explains the following with respect to the US far-right in a complex interaction with the growth of transnational corporations, lobby groups, individuals(2), informal networks and state actors with respect to objects of threat and threat multipliers(3) in connection with the post-bipolar, global anchoring of US economic and consumer patterns: US adaptation of its reaction to this threat – while consolidating imperial presidency(4) and weakening the system of checks and balances – including its implications of a bipolar liberal order. In this way, the necessary continued leadership within NATO through the US-proposed NATO reform can be seen as an appropriate implementation of transformed threat-reaction measures and the legitimisation of systemic adaptation. It equally becomes clear that the established threat reaction measures only provide a short-term defence: instead, they enhance the asymmetric and conventional threat, as well as threat multipliers – by introducing arms races and breaking down arms control – thereby heightening the overall threat. The consequence is the consistently growing likelihood of a conflict of hitherto unimaginable proportions. At the same time, the urgent need to mobilise transatlantic cooperation with respect to supporting global cooperation between state and non-government actors is illustrated with respect to the roots of the threat and its deteriorating underlying conditions: each increase in the overall threat, the adapted US security policy and its continuation in NATO is connected to an erosion of rule-based underlying criteria during the studied period. This continuously and consistently undermines the basis of the above-stated, ever-increasingly important cooperation, to prevent or at least limit the successive erosion of the bipolar “American system” under future dystopias. The research results completely overturn the state of research to date, since for instance it is possible to show that, by means of NATO transformation findings, no transatlantic sharing of burdens on an equal footing and no NATO reform in accordance with its founding principles can be achieved. The same also applies to European opposition to the actual anchoring of NATO transformation positions(5), which is based on the erosion of the bipolar liberal order system and the maintenance of US advantages as well as the consolidation of particular interests they facilitate. Furthermore, it is apparent that a line of continuity in the threat-reaction measures from Clinton to Obama exists with varying external effects, along with an underlying pattern of “Battleship America” – as opposed to a multilaterally orientated foreign and security policy under Clinton, which merged into a unilateral, radical swing under G. W. Bush 43 following 9/11, but was reverted by the Obama administration. A comprehensive wealth of literature was used of the doctoral thesis, as reflected by the extensive bibliography: they firstly include diverse American and European publications, monographs and relevant secondary literature, including biographies, publications of various kinds of important political planning and implementation, as well as collected volumes and research articles from specialist journals on all fields of research and politological methodology and theory. The same applies to publications by leading European and American institutions, research centres and think tanks. Furthermore, this author used publications and documents by governments, foreign ministries, defence ministries, other government bodies and Nato.
Dissertation structure
This dissertation is divided into two volumes and one Appendix: Volume 1 discusses Focus 1, namely a process-tracing in the context of offensive neorealist positioning. Volume 2 presents Focus 2, which is based on the preceding focus in making a structured, focussed comparison in the context of defensive neorealist positioning. The Appendix volume contains further discussion of Chapter 1, Volume 1 with respect to the state of research, literature and sources, theoretical positioning and the choice of the region of study and selected European NATO partners. Furthermore, a historical chapter provides underlying information for process-tracing in Chapter 2, Volume 1, an index of images and abbreviations, and a bibliography.
The entire dissertation uses qualitative methods to focus on these two mutually supporting, building on each other, themes to investigate the following from a US-perspective: firstly the overriding US security-policy reaction to a new overall threat and secondly, its continuation combined with the opportunity of for enabling and legitimising it within and through NATO during the studied period from Clinton to Obama.
Based on the first part of this hypothesis, Focus 1 (Volume 1) establishes a connection between, on the one hand, maintaining the bipolar Grand Strategy target of consolidating the USA as a leading, regulating power, bipolar foreign-policy Grand Strategy indicators and a new overall threat that is developing in a complex way, and, on the other, the necessity of its continued leadership within NATO and the required NATO transformation according to US-proposed NATO transformation positions.
Focus 2 (Volume 2) is based on the second part of the hypothesis, investigating the transatlantic negotiation process to establish these US-proposed NATO transformation positions: in this context, Volume 2 investigates whether the attempt to actually secure and consolidate such US supremacy was unsuccessful in the face of resistance from selected European NATO partners, namely France, Germany and the United Kingdom.
The overall result shows that due to a complex, developing, linear increase in the overall threat, the chance for the USA to consolidate its status as a leading power is steadily diminishing. This must be compensated by adapting US security policy. The resulting American security-policy realignment based on the initiated “revolution in military affairs” in turn modifies the indicators of bipolar collective security guarantees. Everything is enabled and legitimised by means of actually securing US NATO-transformation positions. The actual implementation of such NATO transformation – representing the consistent adaptation of US security policy – enables a mission-orientated, rapid response, flexible, global security projection. It also creates conditions for “alliances of choice” within NATO. Furthermore, the modification of a “bipolar NATO” exacerbates the erosion of key achievements of civilisation as a result of adapted US security policy, as well as undermining the tasks of bipolar collective security guarantees through diminished benefits to European NATO partners.
The actual anchoring of NATO transformation positions is achieved by reactivating the conventional threat in the context of the Ukraine crisis of 2014 and the extension of NATO partnership rings on a global level, without providing them with NATO membership status, thus avoiding globalisation in a mutual defence case. The German and French resistance is particularly intensive through the involvement of European founder states, while the formation of a European leadership triumvirate consisting of France, Germany and the United Kingdom does not take place.
Moreover, a relevant investigation of causes particularly shows that despite constant mutually supporting US security reaction measures with varying international effects and actual continued leadership within NATO, the overall threat is not receding: this leads to a constant increase in the overall threat, a loss of influence of state actors, the diffusion and concentration of power and the increased probability of reactive conventional, nuclear, cyber and ecological destruction scenarios. On this basis, the consequence is an increasingly comprehensive and rapidly responding precision defence combined with growing securitization to compensate for the ongoing containment of US supremacy. This developing process steadily diminishes the reach and power of a liberal, rule-based, bipolar “American system” and the establishment of “idealistic, liberal” elements of US-Grand Strategy. This entails a further reduction in benefits for European NATO allies and increasing US cost-cutting demands – based on the successive NATO transformation positions that build on each other and Obama’s “smart power”(6) during the period studied in this dissertation. Thus the chance is receding of developing the post-bipolar, globally adopted American way of life with individual national character, which is regarded as “non-negotiable”: for instance its articulation is expressed through increasing right-wing populism, the election of outsider-candidates, the dissolution of traditional party systems, isolationist tendencies combined with burgeoning ethnic, regional movements, the rejection of supranationalism, and religious fundamentalism. At the same time, the ongoing erosion of global public goods is apparent.
This all paves the way to limiting the benevolent American regulating power and state actors’ leverage – and therefore to a return to classic power politics in the context of a resulting diffusion and concentration of power. In view of the urgency of a long-term containment of asymmetrical or conventional threats to security, or aspects that exacerbate such threats or clusters thereof, as well as underlying global conditions, this undermines the ability to achieve the following: to achieve transatlantic cooperation by broadening the range of levels and actors in the spirit of proactive and expanded, networked security to achieve according global cooperation with respect to containing the root causes of threats.
Overall, this research work reveals how and why the anticipated “peace dividend” and the notion of an “age of hope”, as postulated by President Clinton, were hardly perceptible during the period of study between 1993 and 2017.
Notes
(1) Cf. Braml, Josef (2018), Trumps transaktionaler Transatlantizismus, in: Jäger, Thomas (Hrsg.), Zeitschrift für Außen- und Sicherheitspolitik, Oktober 2018, Volume 11, Ausgabe 4, S. 439-448, Wiesbaden.
(2) Cf. National Intelligence Council (Ed.) (2012), Global Trends 2013: Alternative Worlds (NIC 2012-001), https://publicintelligence.net/global-trends-2030/, last accessed: 12.04.19. See also the “international financial leadership, self-selected at Davos” cit. McCoy, Alfred W. (2017), In the Shadows of the American Century. The Rise and Decline of US Global Power, Chicago.
(3) In 1990, the threat-enhancing nature of climate change was already postulated with respect to asymmetric objects of threat as well as conventional and complex clusters: “Over the next half century, the global average temperature may increase by approximately 4 degrees C. (…) All nations will be affected. (…) How much time will there be to confirm the amount of change and then to act? (…) However, many believe that we will have waited too long to avoid major dislocation, hardship and conflict – on a scale not as yet seen by man“. Cf. Kelley, Terry P. (1990), Global Climate Change. Implications For The United States Navy (The United States Naval War College, Newport, RI), http://documents.theblackvault.com/documents/weather/climatechange/globalclimatechange-navy.pdf, last accessed: 30.03.19. Cf. Mazo, Jeffrey (2010), Climate Conflict. How global warming threatens security and what to do about it, London, Abingdon.
This supports the thesis of a developing, constant overall threat during the period between 1993 and 2017.
(4) Cf. Schlesinger, Arthur M., Jr. (1973), The Imperial Presidency, Boston.
(5) In this dissertation, the proposed US positions on NATO adaptation, the NATO Response Force and the Global Partnership Initiative are described as “NATO transformation positions”: Their actual establishment was connected to a NATO transformation with the consistent continuation of adapted US security policy.
(6) Cf. Nossel, Suzanne (2004), Smart Power. Reclaiming Liberal Internationalism, http://www.democracyarsenal.org/SmartPowerFA.pdf, last accessed: 26.08.17, Nye, Joseph S. Jr. (2011), The Future of Power, New York, Nye, Joseph S. Jr. (2011), Macht im 21sten Jahrhundert. Politische Strategien für ein neues Zeitalter, München, Rodham Clinton, Hillary (2010), Leading Through Civilan Power. Redefining American Diplomacy and Development, in: Foreign Affairs, November/December 2010, Vol. 89, No.6, S. 13-24.
Die Analyse ortsbezogener Strukturdaten und Merkmale ist eine der wesentlichen Voraussetzungen für die Bewertung von Standorten und Räumen. Durch die Erfassung der lokalen Ausprägung ausgewählter Standortfaktoren und unter Zuhilfenahme eines Geographischen Informationssystems werden in der vorliegenden Dissertation die Städte und Gemeinden des hessischen Rheingau-Taunus-Kreises kategorisiert und miteinander verglichen. Hierbei erfolgt eine besondere Fokussierung auf die lokal bestehende technische und soziale Infrastruktur, um vorrangig das vorhandene Gewerbe- und Wohnumfeld sowie die darüber hinaus existierenden räumlichen Disparitäten eingehend veranschaulichen und beurteilen zu können.
Statistical eco(-toxico)logy
(2017)
Freshwaters are of immense importance for human well-being.
Nevertheless, they are currently facing unprecedented levels of threat from habitat loss and degradation, overexploitation, invasive species and
pollution.
To prevent risks to aquatic ecosystems, chemical substances, like agricultural pesticides, have to pass environmental risk assessment (ERA) before entering the market.
Concurrently, large-scale environmental monitoring is used for surveillance of biological and chemical conditions in freshwaters.
This thesis examines statistical methods currently used in ERA.
Moreover, it presents a national-scale compilation of chemical monitoring data, an analysis of drivers and dynamics of chemical pollution in streams and, provides a large-scale risk assessment by combination with results from ERA.
Additionally, software tools have been developed to integrate different datasets used in ERA.
The thesis starts with a brief introduction to ERA and environmental monitoring and gives an overview of the objectives of the thesis.
Chapter 2 addresses experimental setups and their statistical analyses using simulations.
The results show that current designs exhibit unacceptably low statistical power, that statistical methods chosen to fit the type of data provide higher power and that statistical practices in ERA need to be revised.
In chapter 3 we compiled all available pesticide monitoring data from Germany.
Hereby, we focused on small streams, similar to those considered in ERA and used threshold concentrations derived during ERA for a large-scale assessment of threats to freshwaters from pesticides.
This compilation resulted in the most comprehensive dataset on pesticide exposure currently available for Germany.
Using state-of-the-art statistical techniques, that explicitly take the limits of quantification into account, we demonstrate that 25% of small streams are at threat from pesticides.
In particular neonicotinoid pesticides are responsible for these threats.
These are associated with agricultural intensity and can be detected even at low levels of agricultural use.
Moreover, our results indicated that current monitoring underestimates pesticide risks, because of a sampling decoupled from precipitation events.
Additionally, we provide a first large-scale study of annual pesticide exposure dynamics.
Chapters 4 and 5 describe software solutions to simplify and accelerate the integration of data from ERA, environmental monitoring and ecotoxicology that is indispensable for the development of landscape-level risk assessment.
Overall, this thesis contributes to the emerging discipline of statistical ecotoxicology and shows that pesticides pose a large-scale threat to small streams.
Environmental monitoring can provide a post-authorisation feedback to ERA.
However, to protect freshwater ecosystems ERA and environmental monitoring need to be further refined and we provide software solutions to utilise existing data for this purpose.
Grundwasser ist eine lebenswichtige Ressource und gleichzeitig ein thermisch stabiler Lebensraum mit einer außergewöhnlichen Fauna. Aufgrund der Klimageschichte der letzten ca. 1,8 Millionen Jahre in Mitteleuropa war die Ausgangshypothese, dass die meisten Grundwasserarten an Temperaturen unterhalb von 14 °C angepasst sind und sich Temperaturerhöhungen daher negativ auf die Grundwasserfauna auswirken.
Um die Folgen einer Klimaerwärmung in situ beurteilen zu können, wurden anthropogen erwärmte Grundwässer als Modelle herangezogen. In ersten Teil dieser Arbeit wurden die Crustaceagemeinschaften natürlich temperierter und anthropogen erwärmter Standorte untersucht. Dazu wurden insgesamt 70 Grundwassermessstellen im Oberrheingraben über ein Jahr (2011 bis 2012) sechsmal beprobt. Ergänzend zu diesem synökologischen Ansatz wurde in einem zweiten Teil die Temperaturpräferenz ausgewählter Arten überprüft. Für diese autökologische Betrachtung wurde ein deutschlandweiter Datensatz herangezogen.
Die ausgewählten Untersuchungsgebiete haben sich als geeignete Modelle für die prognostizierten Temperaturerhöhungen herausgestellt. Die Temperatur erwies sich als einer der wichtigsten Parameter für die Crustaceagemeinschaften. Erwärmungen beeinflussen die Zusammensetzung der Gemeinschaften und die Diversität. Dabei zeigte sich, dass es artspezifisch unterschiedliche Temperaturpräferenzen gibt. Einige vermutlich weniger streng stygobionte Arten scheinen höhere Temperaturen zu tolerieren. Der Großteil der stygobionten Grundwasserarten bevorzugt allerdings Temperaturen unterhalb von 14 °C, wobei wenige Arten als „extrem kalt¬steno-therm“ einzustufen sind. Diese Arten wurden an Standorten nachgewiesen, die aufgrund der hydrologischen Verhältnisse relativ kühl sind und der Fauna auch zukünftig als kalte Refugien Schutz bieten könnten. Einer dieser Standorte ist aufgrund seiner Artenvielalt als „Hot Spot“ einzustufen. Die Ergebnisse legen nahe, dass vor allem kaltstenotherme Arten durch eine Klimaerwärmung und anthropogene Wärmeeinträge gefährdet sind.
Die Untersuchung zeigt, dass Grundwasserlebensräume vor Temperaturerhöhungen zu bewahren sind. Lokale anthropogene Wärmeeinträge sollten keine dauerhafte und großflächige Grundwassererwärmung über 14 °C zur Folge haben. Thermische Ein-träge sollten überwacht und bewertet werden. Es wird empfohlen, die bestehenden rechtlichen Rahmenbedingungen den ökologischen Erfordernissen anzupassen.
The European weatherfish (Misgurnus fossilis) is a benthic freshwater fish species belonging to the family Cobitidae, that is subjected to a considerable decline in many regions across its original distribution range. Due to its cryptic behavior and low economic value, the causes of threat to weatherfish remained partly unknown and the species is rarely at the center of conservation efforts. In order to address these concerns, the overall aim of the present thesis was to provide a comprehensive approach for weatherfish conservation, including the development of stocking measures, investigations on the species autecology and the evaluation of potential threats. The first objective was to devise and implement a regional reintroduction and stock enhancement program with hatchery-reared weatherfish in Germany. Within this program (2014-2016), a total number of 168,500 juvenile weatherfish were stocked to seven water systems. Recaptures of 45 individuals at two reintroduction sites supported the conclusion that the developed stocking strategy was appropriate. In order to broaden the knowledge about weatherfish autecology and thereby refining the rearing conditions and the selection of appropriate stocking waters, the second objective was to investigate the thermal requirements of weatherfish larvae. Here, the obtained results revealed that temperatures higher than previously suggested were tolerated by larvae, whereas low temperatures within the range of likely habitat conditions increased mortality rates. As weatherfish can be frequently found in agriculturally impacted waters (e.g. ditch systems), they are assumed to have an increased probability to be exposed to chemical stress. Since the resulting risk has not yet been investigated with a focus on weatherfish, the third objective was to provide a methodical foundation for toxicity testing that additionally complies with the requirements of alternative test methods. For this purpose, the acute fish embryo toxicity test was successfully transferred to weatherfish and first results exhibited that sensitivity of weatherfish towards a tested reference substance (3,4-dichloroaniline) was highest compared to other species. On the basis of these findings, the fourth objective was to apply weatherfish embryos for multiple sediment bioassays in order to investigate teratogenic effects derived from sediment-associated contaminants. In this context, weatherfish revealed particular sensitivity to water extractable substances, indicating that sediment contamination might pose a considerable risk. Moreover, as an endangered benthic fish species with high ecological relevance for European waters that are specifically exposed to hazardous contaminants, the weatherfish might be a prospective species for an ecological risk assessment of sediment toxicity. Overall, the present thesis contributed to the conservation of weatherfish by considering a variety of aspects that interact and reinforce one another in order to achieve improvements for the species situation.
Key mechanisms for the release of metal(loid)s from a construction material in hydraulic engineering
(2017)
Hydraulic engineering and thus construction materials are necessary to enable the navigability of water ways. Since, a variety of natural as well as artificial materials are used, this materials are world wide tested on a potential release of dangerous substances to prevent adverse effects on the environment. To determine the potential release, it is important to identify and to understand key mechanisms which are decisive for the release of hazardous substances. A limited correlation between the conditions used in regulatory tests and those found in environmental systems is given and hence, often the significance of results from standardised tests on construction materials is questioned, since they are not designed to mimic environmental conditions.
In Germany industrial by-products are used as armour stones in hydraulic engineering. Especially the by-product copper slag is used during the last 40 years for the construction of embankments, groynes and coastal protection. On the one hand, this material has a high density and natural resources (landscape) are protected. One the other hand, the material contains high quantities of metal(loid)s. Therefore the copper slag (product name: iron silicate stones) is very suitable as test material. Metal(loid)s examined were As, Sb and Mo as representatives for (hydr)oxide forming elements and Cd, Co, Cu, Fe, Ni, Pb and Zn were studied as representatives for elements forming cations during the release.
Questions addressed in this Thesis were: (i) can we transfer the results from batch experiments to construction scenarios under the prevalent environmental conditions, (ii) which long-term trends exist for the release of metal(loid)s from copper slags and (iii) how environmental conditions influence the leaching of metal(loid)s from water construction materials?
To answer the first question the surface depending release of the metal(loid)s from the construction materials was examined. Therefore, batch leaching experiments with different particle sizes and a constant liquid/solid ratio were performed. In a second step a comparison between different methods for the determination of the specific surface area of armour stones with a 3D laser scanning method as a reference were performed. In a last step it was possible to show that via a roughness factor the results of the specific surface area from small stones, measured with gas adsorption, can be connected with the results from armour stones, determined with an aluminium foil method. Based on calculations of the specific surface area, it was possible to significantly improve catchment scale calculation about the release of metal(loid)s and to evaluate a potential impact of construction materials in hydraulic engineering on the water chemistry of rivers and streams.
To answer the second question long-term leaching diffuse gradient in thin films supported experiments were performed for half a year. Diffuse gradients in thin films (DGT) is an in situ method to passive sample metal(loid)s in water, sediments and soils. They were used as a sink for metal(loid)s in the eluate to provide solution equilibriums. Thus the exchange of the eluent, which is performed normally in long-term experiments, was superfluous and long-term effects under undisturbed conditions were studied. The long-term leaching experiments with DGT have proven to be capable (i) to differentiate between the depletion of the material surface and the solution equilibriums and (ii) to study sorption processes with or without a further release of the analytes. This means for the practically relevant test material copper slag that: (i) the cations Cd, Co, Cu, Ni and Pb are confirmed to be released from the slag over the whole time period of six months, (ii) a surface depletion of Zn was detected, and (iii) that the (hydr)oxide forming elements As, Mo and Sb were released from the slag over the hole periods of six months but the release was masked by adsorption to Fe-oxide colloids, which were formed during the leaching experiments. It was confirmed, that sulphide minerals are the main source for long-term release of Cd, Cu, Ni, Pb and Mo.
To answer the third question short-term leaching experiments simulating environmental conditions in hydraulic engineering were performed. One factor is the salinity. The influence of this parameter was tested in batch experiments with sea salt solution (30 g/l), river Rhine water, ultra pure water and in addition with different NaCl concentration (5, 10, 20 and 30 g/l). In general, the ionic strength is an important factor for the metal(loid) release but the composition of the water (e.g. the HCO3- content) may superimpose this effect. Therefore, the concentrations of the metal(loid)s in the experiments with ultra-pure water spiked with sea salt or native river water and the ultra-pure water spiked with NaCl were significantly different. In a second experiment the influence of the environmental parameters and the interactions between the environmental parameters pH (4–10), sediment content (0 g–3.75 g), temperature (4 °C–36 °C) and ionic strength (0 g/l–30 g/l NaCl) on the release of metal(loid)s from the test material was examined. The statistical Design of Experiments (DoE) was used to study the influence of these factors as well as their interactions. All studied factors may impact the release of metal(loid)s from the test material to the eluent, whereas the release and the partitioning between sediment and eluate of metal(loid)s was impacted by interactions between the studied factors. The main processes were sorption, complexation, solubility, buffering and ion exchange. In addition, by separating the sediment from the slag after the experiments by magnetic separation, the enrichment of metal(loid)s in the sediment was visible. Thus, the sediment was the most important factor for the release of the metal(loid)s, via pH, temperature and ionic strength, because the sediment acted as a sink.
Die deutsche Holzhausbranche wächst seit Jahren, allerdings beschäftigen sich die Unternehmen nicht strategisch mit dem Thema Marketing. Diese Dissertation bildet durch qualitative und quantitative Bewohnerbefragungen die Basis für die Entwicklung eines strategischen Marketingansatzes im Holzhausbereich.
In der vorliegenden Arbeit wird das thermochemische Wechselwirkungsverhalten verschiedener Magnesiakohlenstoffmaterialen in Abhängigkeit verschiedener Einflussgrößen wissenschaftlich untersucht. Schwerpunkte der experimentellen Arbeiten bilden thermoanalytische Experimente, Gefügeuntersuchungen der Magnesiakohlenstoff-Proben sowie thermodyna-mische Berechnungen und Auswertungen durch CAT (Computer Aided Thermochemistry) mittels des Softwarepakets FactSage.
Erster Themenbereich dieser Arbeit ist die Untersuchung des Einflusses der in dem Rohstoff Magnesia enthaltenen mineralogischen Nebenphasen Merwinit (C3MS2), Monticellit (CMS) und Belit (C2S) auf den carbothermisch induzierten Verschleiß im MgO-C-Material. Für die Messreihen wurden die Nebenphasen eigens synthetisiert und hiermit MgO-C-Nebenphase-Modellwerkstoffe hergestellt. Die Nebenphase Monticellit ist unbeständig gegenüber der carbothermischen Reduktion. Monticellit wird im MgO-C-Gefüge durch Kohlenstoff reduziert und hieraus ergibt sich ein erhöhter Gewichtsverlust des Probenmaterials. Auch Merwinit wird bei T = 1600°C reduziert, der Gewichtsverlust wird dadurch allerdings nicht erhöht. Belit ist im MgO-C-Gefüge stabil gegenüber carbothermischer Reduktion.
Ein weiterer Schwerpunkt der Arbeit lag auf der Untersuchung des Einflusses des klassischen Antioxidans Aluminium auf die thermochemische Stabilität von MgO-C. Bei geringen Sauerstoffpartialdrücken ist die Reaktion des Aluminium-Metalls bzw. des bereits zu Al4C3 carbidisierten Aluminiums mit dem steineigenen Periklas unter Mg(g)-Bildung möglich, was einen erhöhten Gewichtsverlust zur Folge hat. Aber auch nach der Oxidation zu Al2O3 bzw. Spinell liegt Aluminium in signifikanten Mengen als Al(g) und Al2O(g) in der Gasphase vor und greift des Weiteren die Nebenphasen an, was ebenfalls zu einem messbaren Gewichtsverlust führt.
Dritter Arbeitsschwerpunkt war die Untersuchung des Einflusses des Umgebungsdruckes auf die carbothermische Reduktion von MgO. Die Ergebnisse zeigen, dass der Druck sich in zweierlei Hinsicht auf die carbothermische Reduktion von MgO auswirkt. Zum einen bewirkt ein sinkender Umgebungsdruck eine Beschleunigung der carbothermischen Reduktion durch die Verschiebung des thermodynamischen Gleichgewichts auf die Produktseite. Des Weiteren sorgt er für einen schnelleren Abtransport der Produktgase vom Reaktionsort und ver-hindert somit die Einstellung eines lokalen Gleichgewichts im Gefüge. Dritter Effekt ist die mit steigendem Druck verstärkt ablaufende Kohlenstoffoxidation durch Umgebungssauerstoff, da die Sauerstoffmenge in der Umgebung des MgO-C-Materials vom Umgebungsdruck bestimmt wird. Für die Geschwindigkeit des thermochemischen Verschleißes von Magnesiakohlenstoffmaterialien, der immer eine Kombination aus Kohlenstoffoxidation und carbothermischer Reduktion darstellt, bedeutet dies, dass sie in Abhängigkeit vom Umgebungsdruck in unterschiedlichem Ausmaß von diesen beiden Reaktionen beeinflusst wird.
Natural pest control and pollination are important ecosystem services for agriculture. They can be supported by organic farming and by seminatural habitats at the local and landscape scale.
The potential of seminatural habitats to support predatory flies (chapters 2 and 3) and bees(chapter 7) at the local and landscape scale was investigated in seminatural habitats. Predatory flies were more abundant in woody habitats and positively related to landscape complexity. The diversity and the abundance of honey and wild bees were positively related to the supply of flowers offered in the seminatural habitats.
The influence of organic farming, adjacent seminatural habitats and landscape complexity on pest control (chapter 4) and pollination (chapter 6) was investigated in 18 pumpkin fields. Organic farming lacked strong effects both on the pest control and on the pollination of pumpkin.
Pest control is best supported at the local scale by the flower abundance in the adjacent habitat. The flower supply positively affected the density of natural enemies and tended to reduce aphid densities in pumpkin fields.
Pumpkin provides a striking example for a dominant role of wild pollinators for pollination success, because bumble bees are the key pollinators of pumpkin in Germany, despite a higher visitation frequency of honey bees. Pollination is best supported by landscape complexity. Bumble bee visits and as a result pollen delivery in pumpkin were negatively related to the dominance of agricultural land in the surrounding landscape.
The influence of aphid density (chapter 8) and pollination (chapter 5) on pumpkin yield was evaluated. Pumpkin yields were not affected by aphid densities observed in the pumpkin fields and not limited by pollination at the current levels of bee visitation.
In conclusion, especially seminatural habitats, that provide diverse, continuous floral resources, are important for natural enemies and pollinators. A sufficient proportion of different seminatural habitat types in agricultural landscapes should be maintained and restored. Thereby natural enemies such as predatory flies, wild pollinators such as bumble bees, and the pest control and pollination provided by them can be supported.
Die Dissertation bietet einen internationalen Vergleich von Gestaltungsmustern der Flexibilitätssteuerung von Unternehmen mit Schwerpunkt auf die Automobilindustrie. Vor dem Hintergrund unterschiedlicher nationaler institutioneller Systeme werden betriebliche Flexibilitätsstrategien analysiert und deren Hintergründe und Funktionsweisen detailliert erläutert. Die Analyse institutioneller Rahmenbedingungen erfolgt mittels der "Varieties of Capitalism". Die Eruierung von Flexibilitätsstrategien basiert auf einer internationalen Fallstudie.
Grassland management has been increasingly intensified throughout centuries since mankind started to control and modify the landscape. Species communities were always shaped alongside management changes leading to huge alterations in species richness and diversity up to the point where land use intensity exceeded the threshold. Since then biodiversity became increasingly lost. Today, global biodiversity and especially grassland biodiversity is pushed beyond its boundaries. Policymakers and conservationists seek for management options which fulfill the requirements of agronomic interests as well as biodiversity conservation alongside with the maintenance of ecosystem processes. However, there is and will always be a trade-off.
Earlier in history, natural circumstances in a landscape mainly determined regionally adapted land use. These regional adaptions shaped islands for many specialist species, and thus diverse species communities, favoring the establishment of a high β-diversity. With the raising food demand, these regional and traditional management regimes became widely unprofitable, and the invention of mineral fertilizers ultimately led to a wide homogenization of grassland management and, as follows, the loss of biotic heterogeneity. In the course of the green revolution, this immediate coherence and the dependency between grassland biodiversity and traditional land use practices becomes increasingly noticed. Indeed, some traditional forms of management such as meadow irrigation have been preserved in a few regions and thus give us the opportunity to directly investigate their long-term relevance for the species communities and ecosystem processes. Traditional meadow irrigation was a common management practice to improve productivity in lowland, but also alpine hay meadows throughout Europe until the 20th century. Nowadays, meadow irrigation is only practiced as a relic in a few remnant areas. In parts of the Queichwiesen meadows flood irrigation goes back to the Middle Ages, which makes them a predestined as a model region to study the long- and short-term effects of lowland meadow irrigation on the biodiversity and ecosystem processes.
Our study pointed out the conservation value of traditional meadow irrigation for the preservation of local species communities as well as the plant diversity at the landscape scale. The structurally more complex irrigated meadows lead to the assumption of a higher arthropod diversity (Orthodoptera, Carabidae, Araneae), which could not be detected. However, irrigated meadows are a significant habitat for moisture dependent arthropod species. In the light of the agronomic potential, flood irrigation could be a way to at least reduce fertilizer costs to a certain degree and possibly prevent overfertilization pulses which are necessarily hazardous to non-target ecosystems. Still, the reestablishment of flood irrigation in formerly irrigated meadows, or even the establishment of new irrigation systems needs ecological and economic evaluation dependent on regional circumstances and specific species communities, at which this study could serve as a reference point.
The presence of anthropogenic chemicals in the natural environment may impact both habitats and human use of natural resources. In particular the contamination of aquatic resources by organic compounds used as pharmaceuticals or household chemicals has become evident. The newly identified environmental pollutants, also known as micropollutants, often have i) unknown ecotoxicological impacts, ii) unknown partitioning mechanisms, e.g. sorption to sediments, and iii) limited regulation to control their emission. Furthermore, like any compound, micropollutants can be transformed while in the environmental matrix to unknown transformation products (TPs), which add to the number of unknown chemicals to consider and thus increase the complexity of risk management. Transformation is at the same time a natural mechanism for the removal of anthropogenic compounds, either by complete degradation (mineralisation) or to innocuous TPs. However, how transformation occurs in real-world conditions is still largely unknown. During the transport of micropollutants from household wastewater to surface water, a large amount of transformation can occur during wastewater treatment—specifically during biological nitrifying–denitrifying treatment processes. The thesis considers the systematic optimisation of laboratory investigative techniques, application of sensitive mass-spectrometry-based analysis techniques and the monitoring of full-scale wastewater treatment plants (WWTPs) to elucidate transformation processes of five known micropollutants.
The first of the five compounds investigated was the antibiotic trimethoprim. Incubation experiments were conducted at different analyte spike concentrations and different sludge to wastewater ratios. Using high-resolution mass spectrometry, a total of six TPs were identified from trimethoprim. The types of TPs formed was clearly influenced by the spike concentration. To the best of our knowledge, such impacts have not been previously described in the literature. Beginning from the lower spike concentration, a relatively stable final TP was formed (2,4-diaminopyrimidine-5-carboxylic acid, DAPC), which could account for almost all of the transformed trimethoprim quantity. The results were compared to the process in a reference reactor. Both by the detection of TPs (e.g., DAPC) and by modelling the removal kinetics, it could be concluded that only experimental results at the low spike concentrations mirrored the real reactor. The limits of using elevated spike concentrations in incubation experiments could thus be shown.
Three phenolic micropollutants, the antiseptic ortho-phenylphenol (OPP), the plastics additive bisphenol A (BPA) and the psychoactive drug dextrorphan were investigated with regard to the formation of potentially toxic, nitrophenolic TPs. Nitrite is an intermediate in the nitrification– denitrification process occurring in activated sludge and was found to cause nitration of these phenols. To elucidate the processes, incubation experiments were conducted in purified water in the presence of nitrite with OPP as the test substance. The reactive species HNO2, N2O3 and the radicals ·NO and ·NO2 were likely involved as indicated by scavenger experiments. In conditions found at WWTPs the wastewater is usually at neutral pH, and nitrite, being an intermediate, usually has a low concentration. By conducting incubation experiments inoculated with sludge from a conventional WWTP, it was found that the three phenolic micropollutants, OPP, BPA and dextrorphan were quickly transformed to biological TPs. Nitrophenolic TPs were only formed after artificial increase of the nitrite concentration or lowering of the pH. However, nitrophenolic-TPs can be formed as sample preparation artefacts through acidification or freezing for preservation, creating optimal conditions for the reaction to take place.
The final micropollutant to be studied was the pain-reliever diclofenac, a micropollutant on the EU-watch list due to ecotoxicological effects on rainbow trout. The transformation was compared in two different treatment systems, one employing a reactor with suspended carriers as a biofilm growth surface, while the other system employed conventional activated sludge. In the biofilm-based system, the pathway was found to produce many TPs each at relatively low concentration, many of which were intermediate TPs that were further degraded to unknown tertiary TPs. In the conventional activated sludge system some of the same reactions took place but all at much slower rates. The main difference between the two systems was due to different reaction rates rather than different transformation pathways. The municipal WWTPs were monitored to verify these results. In the biofilm system, a 10-day monitoring campaign confirmed an 88% removal of diclofenac and the formation of the same TPs as those observed in the laboratory experiments. The proposed environmental quality standard of 0.05 μg/L might thus be met without the need for additional treatment processes such as activated carbon filtration or ozonation.
For a comprehensive understanding of evolutionary processes and for providing reliable prognoses about the future consequences of environmental change, it is essential to reveal the genetic basis underlying adaptive responses. The importance of this goal increases in light of ongoing climate change, which confronts organisms worldwide with new selection pressures and requires rapid evolutionary change to avoid local extinction. Thereby, freshwater ectotherms like daphnids are particularly threatened. Unraveling the genetic basis of local adaptation is complicated by the interplay of forces affecting patterns of genetic divergence among populations. Due to their key position in freshwater communities, cyclic parthenogenetic mode of reproduction and resting propagules (which form biological archives), daphnids are particularly suited for this purpose.
The aim of this thesis was to assess the impact of local thermal selection on the Daphnia longispina complex and to reveal the underlying genetic loci. Therefore, I compared genetic differentiation among populations containing Daphnia galeata, Daphnia longispina and their interspecific hybrids across time, space, and species boundaries. I revealed strongly contrasting patterns of genetic differentiation between selectively neutral and functional candidate gene markers, between the two species, and among samples from different lakes, suggesting (together with a correlation with habitat temperatures) local thermal selection acting on candidate gene TRY5F and indicating adaptive introgression. To reveal the candidate genes’ impact on fitness, I performed association analyses among data on genotypes and phenotypic traits of D. galeata clones from seven populations. The tests revealed a general temperature effect as well as inter-population differences in phenotypic traits and imply a possible contribution of the candidate genes to life-history traits. Finally, utilizing a combined population transcriptomic and reverse ecology approach, I introduced a methodology with a wide range of applications in evolutionary biology and revealed that local thermal selection was probably a minor force in shaping sequence and gene expression divergence among four D. galeata populations, but contributed to sequence divergence among two populations. I identified many transcripts possibly under selection or contributing strongly to population divergence, a large amount thereof putatively under local thermal selection, and showed that genetic and gene expression variation is not depleted specifically in temperature-related candidate genes.
In conclusion, I detected signs of local adaptation in the D. longispina complex across space, time, and species barriers. Populations and species remained genetically divergent, although increased gene flow possibly contributed, together with genotypes recruited from the resting egg bank, to the maintenance of standing genetic variation. Further work is required to accurately determine the influence of introgression and the effects of candidate genes on individual fitness. While I found no evidence suggesting a response to intense local thermal selection, the high resilience and adaptive potential regarding environmental change I observed suggest positive future prospects for the populations of the D. longispina complex. However, overall, due to the continuing environmental degradation, daphnids and other aquatic invertebrates remain vulnerable and threatened.